Custom Panels for Third Generation Sequencing

Range 1 – 0-50.000 bp

The Custom Panels for Third Generation Sequencing reagent developed by iBioScience Ltd. facilitates the implementation of targeted sequencing. Targeted sequencing allows you to focus on smaller regions during sequencing, thereby reducing the cost of sequencing and increasing the depth of sequencing. Using the reagents, regions from 0 to 50,000 bp in length can be covered with approximately 5,000 bp amplicons, which is ideal for sequencing devices with long read lengths. After amplification, the barcode sequences for each amplicon are also transferred to the target molecule consequently no further labeling of the samples is required thereafter.

Custom Panels for Third Generation Sequencing

Range 2 – 50.000-200.000 bp

The Custom Panels for Third Generation Sequencing reagent developed by iBioScience Ltd. facilitates the implementation of targeted sequencing. Targeted sequencing allows you to focus on smaller regions during sequencing, thereby reducing the cost of sequencing and increasing the depth of sequencing. Using the reagents, regions of 50,000 to 200,000 bp in length can be covered with approximately 5,000 bp of amplicons, ideal for sequencing devices with long read lengths. After amplification, the barcode sequences for each amplicon are also transferred to the target molecule consequently no further labeling of the samples is required thereafter.

Custom Panels for Third Generation Sequencing

Range 3 – 200.000-500.000 bp

The Custom Panels for Third Generation Sequencing reagent developed by iBioScience Ltd. facilitates the implementation of targeted sequencing. Targeted sequencing allows you to focus on smaller regions during sequencing, thereby reducing the cost of sequencing and increasing the depth of sequencing. Using the reagents, regions of 200,000 to 500,000 bp in length can be covered with amplicons of approximately 5,000 bp, ideal for sequencing devices with long read lengths. After amplification, the barcode sequences are also transferred to the target molecule for each amplicon consequently no further labeling of the samples is required thereafter.

Third Generation Sequencing Long Amplicon-Seq QC & Validation Kit

Reagent used to optimize quality control and data analysis of libraries for third-generation amplicon-based sequencing used for research purposes.

Third generation sequencing platforms allow amplicons of any length to be read without fragmentation. The TG Long Amplicon Sequencing QC & Validation Kit reagent distributed by iBioScience Ltd. provides the reagents needed to optimize the data analysis parameters for sequencing long amplicons between 2000-5000 bp. The reagent contains known mutations from the PhiX genome (SNPs and INDELs with defined allele frequencies) to facilitate optimized variant calling and screening as a function of coverage. The reagent should be added to the test sample at the final stage of library preparation as an internal control. The oligos in the QC kit contain the barcode sequences used for sequencing, and the motor proteins required for sequencing must be ligated subsequently.

Third Generation Sequencing WGS QC & Validation Kit

Reagent used to optimize quality control and data analysis of third-generation DNA-based whole genome libraries for research purposes.

Third generation sequencing platforms allow reading of a nucleic acid fragment of any length. The TGS WGS Sequencing QC & Validation Kit distributed by iBioScience Ltd. provides the reagents needed to optimize the data analysis parameters in case of complete genome genome sequencing. The reagent contains known mutations from the PhiX virus genome (SNPs and INDELs with defined allele frequencies) to facilitate optimized variant call and screening as a function of coverage. The reagent should be added to the test sample at the final stage of library preparation as an internal control. The oligos in the QC kit contain the barcode sequences used for sequencing, and the motor proteins required for sequencing must be ligated subsequently. The kit contains the software used for data analysis.

NGS WGS QC & Validation Kit

Reagent used for quality control and data analysis optimization for next-generation sequencing DNA-based Whole Genome Sequencing libraries.

The NGS WGS QC & Validation Kit developed by iBioScience Ltd. facilitates the efficient evaluation of whole genome sequencing results from new generation sequencing technology. When identifying somatic variants, it is very important to optimize the data analysis parameters, for example when determining the detection limit with different coverage. Therefore, the NGS WGS QC & Validation Kit is a reagent containing known mutations based on the PhiX genome. Known mutations, SNPs, and short INDELs have a defined allele frequency (1%, 2%, 5%, 10%, 20%, 50%, 75%, and 100%) thus facilitating optimized variant call and filtering as a function of coverage. The reagent should be added to the test sample after library preparation as an internal control as well as sequenced independently as a single clear sample. The oligos in the QC kit already include the universal adapters and barcodes used for sequencing.

NGS Second generation Amplicon-Seq QC & Validation Kit

Reagent to optimize the quality and data analysis of libraries enabling DNA-based targeted region sequencing (Amplicon Sequencing) for research purposes.

The efficient evaluation of the targeted region or amplicon sequencing results is facilitated by the NGS Amplicon-Seq QC & Validation Kit developed by iBioScience Ltd. When identifying somatic variants, it is very important to optimize the data analysis parameters, for example when determining the detection limit with different coverage. Therefore, the NGS Amplicon-Seq QC & Validation Kit is a reagent containing known mutations based on the PhiX genome. Known mutations, SNPs, and short INDELs have a defined allele frequency (1%, 2%, 5%, 10%, 20%, 50%, 75%, and 100%) thus helping optimized variant call and filtering as a function of coverage. The reagent should be added to the test sample after library preparation as an internal control and should be sequenced independently as a unique clear sample, as well. The oligos in the QC kit include the universal adapter and barcodes used for sequencing.

iBio VirSeq ComboKit for SARS-CoV-2 (48 samples per kit)

The kit contains reagents for sequencing the SARS-CoV-2 virus RNA genome for sample preparation and sequencing. The starting sample is viral RNA which can be prepared by cDNA transcription as a first step. The entire SARS-CoV-2 genome must be amplified with specific primers to generate amplicons between 100 and 260 bp in size that cover the entire viral genome. Following amplification, the index sequences are added to the sequence in a PCR reaction using 384 different indices. It is recommended that libraries be sequenced on Illumina platform with 2 × 150 PE reading.

2019-nCoV Enrichment Primer Mix

The 2019-nCoV Enrichment Primer Mix reagent, developed by iBioScience Ltd. and used in new generation sequencing technology, facilitates the efficient determination of the genome of the SARS-CoV2 virus. Because non-viral (human, bacterial) nucleic acid is highly present in addition to viral RNA, an enrichment step is strongly recommended during the sequencing process. The 2019-nCoV Enrichment Primer Mix contains a total of 218 primers that can be used to amplify the entire viral genome by conventional endpoint PCR. By optimizing the concentrations of the primers, balanced primer mixtures can be used to increase the amplicon population to ensure adequate coverage of the entire genome of the virus, even from samples with low viral copy numbers.

NGS RNA-Seq QC & Validation Kit

A new generation sequencing reagent used for research purposes to control the quality of RNA-based sequencing libraries and to optimize data analysis. The NGS RNA-Seq QC & Validation Kit for new generation sequencing technology developed by iBioScience Ltd. facilitates the efficient evaluation of the target region or amplicon sequencing results. During RNA sequencing, it is very important to optimize data analysis parameters, such as determining mapping thresholds. Therefore, the NGS RNA-Seq QC & Validation Kit is a reagent containing known mutations based on the PhiX genome. Known mutations, SNPs, and short INDELs have a defined allele frequency (1%, 2%, 5%, 10%, 20%, 50%, 75%, and 100%) thus facilitating optimized mapping settings and filtering depending on the coverage. The reagent should be added to the test sample after library preparation as an internal control and sequenced independently as a single clear sample, as well. The oligos in the QC kit include the universal adapter used for sequencing and the barcodes.

iBio MagBind RNA Combo 1152 kit

The iBio Magbind RNA Combo 1152 kit contains reagents for automated RNA extraction from viruses. The starting sample is viral transport medium from which RNA can be eluted in 50-100 ul of water after lysis and magnetic bead purification. The contents of the package (robotic consumables, reagents, plastic goods) are compatible with the Hamilton STAR and STARlet fluid handling systems. The kit is suitable for isolation from SARS-CoV-2 virus and for automated COVID-19 diagnostic tests.

KASP (Competitive Allele Specific PCR) is a uniplex PCR-based SNP genotyping procedure that has several advantages over multiplex methods: lower lead time, lower genotype error rate (0.7-1.6%), greater flexibility. It can also be used in cases where many SNP markers need to be tested on a small number of samples or when few SNP markers need to be tested on a lafrge number of samples. During genotyping, samples are taken and identified by barcode, followed by assay design, oligo synthesis, assay assembly and validation. The DNAs of the samples are then normalized and prepared for KASP SNP genotyping. Dilution of the samples and transfer of the 384/1536 PCR microplate is performed using a replicator fluid handling robot. The KASP assay and reaction mix are prepared using the Meridian automated feeder. After sealing the microplate with a Fusion laser foil, the PCR assay is performed on a HydroCycler 64X1536 PCR instrument. This is followed by fluorescence detection with a BMG Labtech FluoStar Omega microplate reader. Data are analyzed and assessed using the Kraken software. The data are evaluated by the Senior Project Manager and subsequently returned to the customer.

Vibration-free Instrument Table

Vibration-free tables based on the principle of passive mechanics for the following Illumina NGS devices:

  • Illumina iSeq100
  • Illumina MiniSeq
  • Illumina MiSeq
  • Illumina NextSeq500/550